Yesterday, I treated the tank with a small amount of Prime (about 1/2 dose by tank volume) due to an increase in ammonia levels.  I did the same thing tonight after learning that the levels were still high (<0.50 ppm total ammonia).

According to Seachem, Prime dissipates from the system 24 hours after treating it, and ammonia tests are accurate again.  I don’t know how true this statement is, or if Seachem just wants you to use more of their product, but as of right now I see no signs of increased ammonia in the water in my fish.  I have an API salicylate-based ammonia testing kit, which you can apparently use with Prime as long as you take the reading right away.

…so far so good!